Part:BBa_K3165038

Gp2 under PcpcG2 Expression

This part places the bacteriostatic growth-inhibitory protein, Gp2 (BBa_K3165007) under the inducible promoter: PcpcG2 (BBa_K2012015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for a bacteriostatic growth-inhibitory protein, Gp2 under an inducible promoter PcpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein.
Upon light stimulation, the production of Gp2 is triggered leading to a bacteriostatic effect. On withdrawal of the light stimuli, the production of Gp2 is hindered and owing to its low half-life, the Gp2 already present in the cells is also degraded.
This part is used in association with the CcaS-CcaR system as a light-induced growth regulation system.